Genetic and molecular characterization of the I locus of Phaseolus vulgaris

Show simple item record Sakiyama, Ney S. Vallejos, C. Eduardo Astua-Monge, Gustavo Jones, Valerie Plyler, Tammy R. Mackenzie, Sally A. 2019-03-07T17:23:14Z 2019-03-07T17:23:14Z 2006-02
dc.identifier.issn 1943-2631
dc.description.abstract The I locus of the common bean, Phaseolus vulgaris, controls the development of four different phenotypes in response to inoculation with Bean common mosaic virus, Bean common mosaic necrosis virus, several other related potyviruses, and one comovirus. We have generated a high-resolution linkage map around this locus and have aligned it with a physical map constructed with BAC clones. These clones were obtained from a library of the cultivar “Sprite,” which carries the dominant allele at the I locus. We have identified a large cluster of TIR–NBS–LRR sequences associated within this locus, which extends over a distance >425 kb. Bean cultivars from the Andean or Mesoamerican gene pool that contain the dominant allele share the same haplotypes as revealed by gel blot hybridizations with a TIR probe. In contrast, beans with a recessive allele display simpler and variable haplotypes. A survey of wild accessions from Argentina to Mexico showed that this multigene family has expanded significantly during evolution and domestication. RNA gel blot analysis indicated that the TIR family of genes plays a role in the response to inoculations with BCMV or BCMNV. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Genetics pt-BR
dc.relation.ispartofseries Volume 172 Issue 2, Pages 1229–1242, February 2006 pt-BR
dc.rights Genetics Society of America pt-BR
dc.subject Genetic and molecular pt-BR
dc.subject Phaseolus vulgaris pt-BR
dc.title Genetic and molecular characterization of the I locus of Phaseolus vulgaris en
dc.type Artigo pt-BR

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    Artigos Técnico-científicos na área de Fitotecnia

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