Characterization of CRISPR-Cas systems in the Ralstonia solanacearum species complex

Show simple item record Xavier, André da Silva Almeida, Juliana Cristina Fraleon de Melo, Alessandra Gonçalves de Rousseau, Geneviève M. Tremblay, Denise M. Rezende, Rafael Reis de Moineau, Sylvain Alfenas‐Zerbini, Poliane 2019-03-14T18:06:29Z 2019-03-14T18:06:29Z 2019-02
dc.identifier.issn 13643703
dc.description.abstract Clustered regularly interspaced short palindromic repeats (CRISPRs) are composed of an array of short DNA repeat sequences separated by unique spacer sequences that are flanked by associated (Cas) genes. CRISPR-Cas systems are found in the genomes of several microbes and can act as an adaptive immune mechanism against invading foreign nucleic acids, such as phage genomes. Here, we studied the CRISPRCas systems in plant-pathogenic bacteria of the Ralstonia sola- nacearum species complex (RSSC). A CRISPR-Cas system was found in 31% of RSSC genomes present in public databases. Specifically, CRISPR-Cas types I-E and II-C were found, with I-E being the most common. The presence of the same CRISPRCas types in distinct Ralstonia phylotypes and species suggests the acquisition of the system by a common ancestor before Ralstonia species segregation. In addition, a Cas1 phylogeny (I-E type) showed a perfect geographical segregation of phylotypes, supporting an ancient acquisition. Ralstonia solanacearum strains CFBP2957 and K60 T were challenged with a virulent phage, and the CRISPR arrays of bacteriophage insensitive mutants (BIMs) were analysed. No new spacer acquisition was detected in the analysed BIMs. The functionality of the CRISPR-Cas interference step was also tested in R. solanacearum CFBP2957 using a spacer-protospacer adjacent motif (PAM) delivery system, and no resistance was observed against phage phiAP1. Our results show that the CRISPR-Cas system in R. solanacearum CFBP2957 is not its primary antiviral strategy. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Molecular Plant Pathology pt-BR
dc.relation.ispartofseries Volume 20, Issue 02, Pages 223-239, February 2019 pt-BR
dc.rights Open Access pt-BR
dc.subject Adaptive immunity pt-BR
dc.subject Transcriptional control pt-BR
dc.subject Bacteria-virus interaction pt-BR
dc.title Characterization of CRISPR-Cas systems in the Ralstonia solanacearum species complex en
dc.type Artigo pt-BR

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  • Artigos [265]
    Artigos Técnico-científicos na área de Microbiologia

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