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Methods for Cercospora coffeicola protoplast isolation and genetic transformation with the green fluorescent protein

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dc.contributor.author Souza, André Gomes Coelho
dc.contributor.author Herrero, Sonia
dc.contributor.author Maffia, Luiz Antonio
dc.contributor.author Daub, Margaret Elizabeth
dc.date.accessioned 2019-03-21T18:10:13Z
dc.date.available 2019-03-21T18:10:13Z
dc.date.issued 2014-06
dc.identifier.issn 1573-8469
dc.identifier.uri https://doi.org/10.1007/s10658-013-0301-9
dc.identifier.uri http://www.locus.ufv.br/handle/123456789/24076
dc.description.abstract Cercospora coffeicola is the causal agent of brown eye spot on coffee leaves. Although the disease has significant importance, few molecular studies have been done with C. coffeicola. Here we report a protocol for isolating protoplasts as well as development of a genetic transformation system using Green Fluorescent Protein. High yields of protoplasts (≈108/ml) were obtained from mycelial cultures from five isolates of C. coffeicola. One isolate was transformed with a vector encoding hygromycin resistance and Green Fluorescent Protein. Out of 43 hygromycin-resistant transformants obtained, Green Fluorescent Protein was highly expressed in one (2.3 %). en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher European Journal of Plant Pathology pt-BR
dc.relation.ispartofseries Volume 139, Issue 2, Pages 241–244, June 2014 pt-BR
dc.rights KNPV pt-BR
dc.subject Brown eye spot pt-BR
dc.subject Coffea arabica pt-BR
dc.subject L. Leaf spot pt-BR
dc.title Methods for Cercospora coffeicola protoplast isolation and genetic transformation with the green fluorescent protein en
dc.type Artigo pt-BR


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  • Artigos [409]
    Artigos Técnico-científicos na área de Fitopatologia

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