Antioxidant study indicative of antibacterial and antimutagenic activities of an ellagitannin-rich aqueous extract from the leaves of Miconia latecrenata

Show simple item record Diaz, Marisa Alves Nogueira Fietto, Luciano Gomes Leite, João Paulo Viana Gontijo, Douglas Costa Gontijo, Pablo Costa Brandão, Geraldo Célio Oliveira, Alaíde Braga de 2019-04-02T11:40:03Z 2019-04-02T11:40:03Z 2019-05-23
dc.identifier.issn 0378-8741
dc.description.abstract Ethnopharmacological relevance; Several plant species of Miconia genus are commonly used in Brazilian folk medicine as anti-inflammatory agents and for the treatment of infectious diseases. Infusions and extracts of Miconia species are also reported as analgesic, antimicrobial, antimalarial, antioxidant, anti-inflammatory, antinociceptive, antimutagenic, and antitumoral. To determine the phytochemical composition of an aqueous extract of Miconia latecrenata leaves and to evaluate its antioxidant, antibacterial, antimutagenic and antigenotoxic activities. Materials and Methods; The following methods were used for the different effects: I) antioxidant - β-carotene/linoleic acid, lipid peroxidation, and DPPH• radical scavenging; II) antibacterial - agar well diffusion and MIC methods); III) antimutagenic assays - Ames Test; and IV) antigenotoxic - Plasmid cleavage test. The phytochemical analysis and phenolic quantification were carried out by UPLC-DAD-ESI-MS/MS and colorimetry, respectively. In addition, statistical correlation analysis was performed aiming to evaluate the Pearson correlation betweenphenolic compounds and biological assays. A high content of tannins was observed and the ellagitannin isomers of 1,2,3,5-tris-galloyl-4,6-HHDP-glucose were identified as the main constituents of the leaves aqueous extract. High antioxidant effect, in different tests, highantibacterial activity to gram-positive and negative strains, as well as high antimutagenic activity were observed. Statistical analysis showed a high Pearson correlation for the tannin content in relation to the results of the antioxidant and antibacterial tests. In general, the antioxidant action of the aqueous extract showed low correlation with the antimutagenic activity. The present results confirmed the expectations regarding the pharmacological profile of M. latecrenata supporting its therapeutic potential in relation to ROS/RNS related disorders. Furthermore, the phenolic compounds of M. latecrenata can act, in turn, minimizing or inhibiting the biological macromolecules damage, especially DNA. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Journal of Ethnopharmacology pt-BR
dc.relation.ispartofseries Volume 236, Pages 114-123, May 2019 pt-BR
dc.rights Elsevier B. V. pt-BR
dc.subject Melastomataceae pt-BR
dc.subject ROS/RNS pt-BR
dc.subject Phenolic compounds pt-BR
dc.subject LC-MS pt-BR
dc.subject DNA protection pt-BR
dc.subject Bio-statistical analysis pt-BR
dc.title Antioxidant study indicative of antibacterial and antimutagenic activities of an ellagitannin-rich aqueous extract from the leaves of Miconia latecrenata en
dc.type Artigo pt-BR

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    Artigos Técnico-científicos nas áreas de Bioquímica e Biologia Molecular

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