Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/25833
Tipo: Artigo
Título: Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping
Autor(es): Bueno, Rafael Delmond
God, Pedro Ivo Vieira Good
Prata, Isadora Oliveira
Pereira, Pedro Henrique Scarpelli
Teixeira, Arlindo Inês
Piovesan, Newton Deniz
Barros, Everaldo Gonçalves de
Abstract: The development of molecular markers is essential for improvement of soybean cultivars with modified fatty acid content. The objective of this study was to identify and validate SNP markers in candidate genes for fatty acid content in soybean. Six candidate genes (ARAF, PDAT, ABI3, FAD2-1b, FAD3B, and FAD3C) were selected. Alignment of gene sequences identified 25 SNPs and 3 INDELs. TSP primers were used to identify SNP alleles. 259 recombinant inbred lines (RILs) (FA22 / CD219) and 185 F2 progenies (A29 / Tucunaré) were tested for association of SNPs. An SNP for FAD3B was associated with variation in content of linoleic acid (R2 = 5.84%) and linolenic acid (R2 = 6.79%). In FAD3C, an SNP was associated with linoleic and linolenic acids (R2 of 9.21% and 18.51%, respectively). The ABI3 gene was associated with palmitic acid, with R2 = 5.41%. The SNP markers identified will be used in assisted selection for improvement of fatty acid content.
Palavras-chave: Low linolenic acid
MAS- marker- assisted selection
SNP- single nucleotide polymorphism
Editor: Crop Breeding and Applied Biotechnology
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1590/1984-70332018v18n3a37
http://locus.ufv.br//handle/123456789/25833
Data do documento: Jul-2018
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
artigo.pdftexto completo383,54 kBAdobe PDFVisualizar/Abrir


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.