Locus  

Effect of neem seed extracts on the development of the Soybean Cysts Nematode

Show simple item record

dc.contributor.author Silva, Júlio C. T.
dc.contributor.author Oliveira, Rosângela D. L.
dc.contributor.author Jham, Gulab N.
dc.contributor.author Aguiar, Naylor D. C.
dc.date.accessioned 2019-07-03T13:29:37Z
dc.date.available 2019-07-03T13:29:37Z
dc.date.issued 2008-05
dc.identifier.issn 1983-2052
dc.identifier.uri http://dx.doi.org/10.1590/S1982-56762008000300001
dc.identifier.uri http://locus.ufv.br//handle/123456789/26053
dc.description.abstract The effect produced by neem seed extracts and a neem seed concentrate (NSC) on the mortality and development of Heterodera glycines was evaluated. Second-stage juvenile (J2) mortality produced by the extracts and NSC was determined. Soybean seedlings treated with each of the extracts (aqueous, methanolic and hexane) were inoculated with J2 and the number of females and eggs per root system determined after 30 days. Both the hexane extracts were ineffective as no statistical difference (P>0.05) in J2 mortality was observed between them and the control. No statistical difference (P>0.05) in J2 mortality (>98%) was obtained between the water extracts and methanol extract at 1000 mg L-1 treatments. The J2 percentage of mortality exponentially increased with the NSC dosages (R2=0.94). LD50 was 42.6 mg L-1 for NSC on H. glycines. In the greenhouse experiment, the highest reduction in the number of females was 84%, obtained with the aqueous (41.6 mg L-1) and methanolic extracts (1000 mg L-1). In the same way, the highest reduction in the number of eggs, of about 90%, was obtained with the same extracts. Thus, using aqueous extracts is more suitable than the remaining extracts in field conditions. The activities of the methanol and NSC extracts were attributed to the seven tetranortritepenoids (azadirachtin H, azadirachtin A, azadirachtin B, desacetylnimbin, desacetylsalannin, nimbin and salannin) identified by reverse phase high performance liquid chromatography. en
dc.description.abstract O efeito dos extratos de sementes de nim e do extrato de sementes concentrado (NSC) foi avaliado na mortalidade e desenvolvimento de Heterodera glycines. A mortalidade de juvenis de segundo estádio (J2) foi avaliada na presença dos extratos de sementes (aquosos, metanólicos e hexânicos) e NCS in vitro. Em casa de vegetação, o número de fêmeas e de ovos por sistema radicular foi determinado 30 dias após a inoculação de plantas de soja com J2 tratados com os extratos de sementes. Os extratos hexânicos foram ineficientes em matar os J2, conquanto nenhuma diferença estatística (P>0.05) foi observada entre as doses estudadas e a testemunha. Os extratos aquosos e o metanólico a 1000 mg L-1 causaram a morte de pelo menos 98% dos J2, mas não houve diferença significativa entre estes tratamentos. A mortalidade dos J2 tratados com NSC cresceu exponencialmente com o aumento das doses (R2=0.94). O LD50 do NSC sobre J2 de H. glycines foi de 42.6 mg L-1. Em casa de vegetação, as maiores reduções no número de fêmeas, 84%, foram obtidas com o extrato aquoso (41.6 mg L-1) e metanólico (1000 mg L-1). Da mesma forma, as maiores reduções no número de ovos, cerca de 90%, foram obtidas com os mesmos extratos. Considerou-se que o uso do extrato aquoso em condições de campo é mais apropriado que os demais extratos. As atividades do extrato metanólico e do NSC foram atribuídas a sete tetranortriterpenóides (azadiractina H, azadiractina A, azadiractina B, desacetilnimbim, desacetilsalanim, nimbim and salanim) que foram identificados por cromatografia líquida de alta performance de fase reversa. pt-BR
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Tropical Plant Pathology pt-BR
dc.relation.ispartofseries v. 33, n. 3, p. 171- 179, mai./ jun. 2008 pt-BR
dc.rights Open Access pt-BR
dc.subject Azadirachta indica pt-BR
dc.subject Heterodera glycines pt-BR
dc.subject Tetranortriterpenoids pt-BR
dc.subject Control pt-BR
dc.subject Nematóide de cistos da soja pt-BR
dc.subject Controle pt-BR
dc.subject Tetranortriterpenóides pt-BR
dc.title Effect of neem seed extracts on the development of the Soybean Cysts Nematode en
dc.type Artigo pt-BR


Files in this item

This item appears in the following Collection(s)

  • Artigos [409]
    Artigos Técnico-científicos na área de Fitopatologia

Show simple item record

Search DSpace


Browse

My Account