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Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between Penicillium expansum and Penicillium griseoroseum

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dc.contributor.author Queiroz, Marisa Vieira de
dc.contributor.author Lana, Taís Guimarães
dc.contributor.author Gonçalves, Daniel Bonoto
dc.contributor.author Araújo, Elza Fernandes de
dc.contributor.author Brito, Admilson Toscano Ribeiro de
dc.contributor.author Varavallo, Maurilio Antonio
dc.date.accessioned 2019-09-26T14:36:49Z
dc.date.available 2019-09-26T14:36:49Z
dc.date.issued 2007-01
dc.identifier.issn 1678-4405
dc.identifier.uri http://dx.doi.org/10.1590/S1517-83822007000100011
dc.identifier.uri https://locus.ufv.br//handle/123456789/27167
dc.description.abstract Protoplast fusion between complementary auxotrophic and morphological mutant strains of Penicillium griseoroseum and P. expansum was induced by polyethylene glycol and calcium ions (Ca2+). Fusant strains were obtained in minimal medium and a prototrophic strain, possibly diploid, was chosen for haplodization with the fungicide benomyl. Different recombinant strains were isolated and characterized for occurrence of auxotrophic mutations and pectinolytic enzyme production. The fusant prototrophic did not present higher pectinase production than the parental strains, but among 29 recombinants analyzed, four presented enhanced enzyme activities. The recombinant RGE27, which possesses the same auxotrophic and morphologic mutations as the P. griseoroseum parental strain, presented a considerable increase in polygalacturonase (3-fold) and pectin lyase production (1.2-fold). en
dc.description.abstract Fusões de protoplastos entre linhagens mutantes auxotróficas e morfológicas complementares de Penicillium griseoroseum e P. expansum foram induzidas por polietilenoglicol e íons cálcio (Ca2+). Fusionantes foram obtidos em meio mínimo e uma linhagem prototrófica, possivelmente diplóide, foi selecionada para a haploidização com o fungicida benomil. Diferentes linhagens recombinantes foram isoladas e caracterizadas quanto à presença de mutações auxotróficas e a produção de enzimas pectinolíticas. O fusionante prototrófico não apresentou maior atividade de pectinases em relação às linhagens parentais, entretanto, entre 29 recombinantes analisados, quatro apresentaram maiores atividades enzimáticas. O recombinante RGE27, o qual possui as mesmas mutações auxotróficas e morfológicas que a linhagem parental de P. griseoroseum, apresentou um aumento considerável na produção de poligalacturonase (3 vezes) e de pectina liase (1,2 vezes). pt-BR
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Brazilian Journal of Microbiology pt-BR
dc.relation.ispartofseries v. 38, n. 01, p. 52- 57, jan./ mar. 2007 pt-BR
dc.rights Open Access pt-BR
dc.subject Protoplast fusion pt-BR
dc.subject Pectinases pt-BR
dc.subject Penicillium pt-BR
dc.subject Fusão de protoplastos pt-BR
dc.title Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between Penicillium expansum and Penicillium griseoroseum en
dc.type Artigo pt-BR


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  • Artigos [268]
    Artigos Técnico-científicos na área de Microbiologia

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