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Campo DC | Valor | Idioma |
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dc.contributor.author | Fialho, Lílian da Silva | |
dc.contributor.author | Guimarães, Valéria Monteze | |
dc.contributor.author | Callegari, Carina Marin | |
dc.contributor.author | Reis, Angélica Pataro | |
dc.contributor.author | Barbosa, Daianny Silveira | |
dc.contributor.author | Borges, Eduardo Euclydes de Lima | |
dc.contributor.author | Moreira, Maurilio Alves | |
dc.contributor.author | Rezende, Sebastião Tavares de | |
dc.date.accessioned | 2018-04-24T11:47:15Z | |
dc.date.available | 2018-04-24T11:47:15Z | |
dc.date.issued | 2008-10-02 | |
dc.identifier.issn | 00319422 | |
dc.identifier.uri | https://doi.org/10.1016/j.phytochem.2008.08.017 | |
dc.identifier.uri | http://www.locus.ufv.br/handle/123456789/19072 | |
dc.description.abstract | Tachigali multijuga Benth. seeds were found to contain protein (364 mg g−1 dwt), lipids (24 mg g−1 dwt), ash (35 mg g−1 dwt), and carbohydrates (577 mg g−1 dwt). Sucrose, raffinose, and stachyose concentrations were 8.3, 3.0, and 11.6 mg g−1 dwt, respectively. α-Galactosidase activity increased during seed germination and reached a maximum level at 108 h after seed imbibition. The α-galactosidase purified from germinating seeds had an Mr of 38,000 and maximal activity at pH 5.0–5.5 and 50 °C. The enzyme was stable at 35 °C and 40 °C, but lost 79% of its activity after 30 min at 50 °C. The activation energy (Ea) values for p-nitrophenyl-α-d-galactopyranoside (pNPGal) and raffinose were 13.86 and 4.75 kcal mol−1, respectively. The Km values for pNPGal, melibiose, raffinose, and stachyose were 0.45, 5.37, 39.62 and 48.80 mM, respectively. The enzyme was sensitive to inhibition by HgCl2, SDS, AgNO3, CuSO4, and melibiose. d-Galactose was a competitive inhibitor (Ki = 2.74 mM). In addition to its ability to hydrolyze raffinose and stachyose, the enzyme also hydrolyzed galactomannan. | en |
dc.format | pt-BR | |
dc.language.iso | eng | pt-BR |
dc.publisher | Phytochemistry | pt-BR |
dc.relation.ispartofseries | v. 69, Issue 14, p. 2579-2585, October 2008 | pt-BR |
dc.rights | Elsevier Ltd. | pt-BR |
dc.subject | Tachigali multijuga benth | pt-BR |
dc.subject | Fabaceae | pt-BR |
dc.subject | Seed germination | pt-BR |
dc.subject | α-Galactosidase | pt-BR |
dc.subject | Galactomannans | pt-BR |
dc.subject | Raffinose family oligosaccharides | pt-BR |
dc.title | Characterization and biotechnological application of an acid α-galactosidase from Tachigali multijuga Benth. seeds | en |
dc.type | Artigo | pt-BR |
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