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https://locus.ufv.br//handle/123456789/22923
Tipo: | Artigo |
Título: | Detection of bovine herpesvirus 1 in cumulus-oocyte complexes of cows |
Autor(es): | Queiroz-Castro, Vanessa L. D. Costa, Eduardo P. da Alves, Saullo Vinicius P. Silva Júnior, Abelardo Machado-Neves, Mariana Guimarães, José D. |
Abstract: | Bovine herpesvirus 1 (BoHV-1) is the causative agent of infectious bovine rhinotracheitis (IBR) and is also associated with reproductive failure. This study investigated the presence of BoHV-1 in cumulus-oocyte complexes (COCs) of naturally-infected cows without clinical signs of IBR. The presence of BoHV-1 in COCs was evaluated by immunofluorescence using confocal laser scanning microscopy. Blood samples and ovaries from 82 cows that had not been vaccinated against BoHV-1 were collected for serological analysis. COCs were divided into two pools: COCs derivate from seropositive cows and from seronegative cows. Then, the samples were processed for confocal microscopy analysis. The results indicated that 61% (50/82) of cows were seropositive for BoHV-1. A total of 719 COCs were obtained from the cows and processed. None of 276 COCs from the 32 seronegative cows presented BoHV-1. However, BoHV-1 was present in the cytoplasm of cumulus cells from 158 out of 443 COCs aspirated from the seropositive cows. The detection of BoHV-1 in the COCs of seropositive cows suggests that the COCs of naturally-infected, asymptomatic cows may be infected with BoHV-1. |
Palavras-chave: | Bovine herpesvirus Cumulus cells Immunolocalization Oocyte |
Editor: | Research in Veterinary Science |
Tipo de Acesso: | 2018 Elsevier Ltd. All rights reserved. |
URI: | https://doi.org/10.1016/j.rvsc.2018.08.010 http://www.locus.ufv.br/handle/123456789/22923 |
Data do documento: | Out-2018 |
Aparece nas coleções: | Artigos |
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artigo.pdf Until 2100-12-31 | Texto completo | 511,12 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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