Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/23252
Tipo: Artigo
Título: Pre‐and post‐puberty expression of genes and proteins in the uterus of Bos indicus heifers: the luteal phase effect post‐puberty
Autor(es): Fortes, M. R. S.
Zacchi, L. F.
Nguyen, L. T.
Raidan, F.
Weller, M. M. D. C. A.
Choo, J. J. Y.
Reverter, A.
Rego, J. P. A.
Boe- Hansen, G. B.
Porto- Neto, L. R.
Lehnert, S. A.
Cánovas, A.
Schulz, B. L.
Islas- Trejo, A.
Medrano, J. F.
Thomas, M. G.
Moore, S. S.
Abstract: Progesterone signaling and uterine function are crucial in terms of pregnancy establishment. To investigate how the uterine tissue and its secretion changes in relation to puberty, we sampled tissue and uterine fluid from six pre- and six postpubertal Brahman heifers. Post-pubertal heifers were sampled in the luteal phase. Gene expression of the uterine tissue was investigated with RNA-sequencing, whereas the uterine fluid was used for protein profiling with mass spectrometry. A total of 4034 genes were differentially expressed (DE) at a nominal P-value of 0.05, and 26 genes were significantly DE after Bonferroni correction (P < 3.1 9 10 À6 ). We also identified 79 proteins (out of 230 proteins) that were DE (P < 1 9 10 À5 ) in the uterine fluid. When we compared proteomics and transcriptome results, four DE proteins were identified as being encoded by DE genes: OVGP1, GRP, CAP1 and HBA. Except for CAP1, the other three had lower expression post-puberty. The function of these four genes hypothetically related to preparation of the uterus for a potential pregnancy is discussed in the context of puberty. All DE genes and proteins were also used in pathway and ontology enrichment analyses to investigate overall function. The DE genes were enriched for terms related to ribosomal activity. Transcription factors that were deemed key regulators of DE genes are also reported. Transcription factors ZNF567, ZNF775, RELA, PIAS2, LHX4, SOX2, MEF2C, ZNF354C, HMG20A, TCF7L2, ZNF420, HIC1, GTF3A and two novel genes had the highest regulatory impact factor scores. These data can help to understand how puberty influences uterine function.
Palavras-chave: Beef cattle
RNA- sequencing
Gene expression
Genetics
Proteomics
Editor: Animal Genetics
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1111/age.12721
http://www.locus.ufv.br/handle/123456789/23252
Data do documento: Dez-2018
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
artigo.pdftexto completo1,2 MBAdobe PDFVisualizar/Abrir


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.