Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/18367
Tipo: Artigo
Título: Genetic evaluation of IS900 partial sequence of Mycobacterium avium subsp. paratuberculosis Brazilian isolates from bovine milk
Autor(es): Carvalho, Isabel Azevedo
Silva, Victor Oliveira
Vidigal, Pedro Marcus Pereira
Silva Junior, Abelardo
Moreira, Maria Aparecida Scatamburlo
Abstract: Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis. Insertion sequence IS900 is used for the identification of MAP. The objective of this study was to verify the genetic conservation of IS900 sequences in raw milk samples. To evaluate genetic conservation, 206 quarter milk samples and 16 bulk-tank milk samples were collected. DNA extraction and IS900 PCR were performed in all samples. Six samples amplified the expected fragment. To confirm the identity of the amplified fragments, PCR products were cloned and sequenced. The resulting sequences were compared with other MAP sequences from GenBank, and it was possible to identify eight polymorphic regions and to form five distinct haplotypes. The number of mutations in each haplotype was verified. IS900 sequence is a very well-conserved sequence that could be used as tool for the molecular detection of this agent and epidemiological purposes. The results showed the first genetic analysis on Brazilian isolates of MAP.
Palavras-chave: Mycobacterium avium subspecies paratuberculosis
Johne’s disease
IS900-PCR
Milk
Editor: Tropical Animal Health and Production
Tipo de Acesso: Springer Science+Business Media B.V.
URI: https://doi.org/10.1007/s11250-012-0117-1
http://www.locus.ufv.br/handle/123456789/18367
Data do documento: 16-Mar-2012
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