Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19072
Tipo: Artigo
Título: Characterization and biotechnological application of an acid α-galactosidase from Tachigali multijuga Benth. seeds
Autor(es): Fialho, Lílian da Silva
Guimarães, Valéria Monteze
Callegari, Carina Marin
Reis, Angélica Pataro
Barbosa, Daianny Silveira
Borges, Eduardo Euclydes de Lima
Moreira, Maurilio Alves
Rezende, Sebastião Tavares de
Abstract: Tachigali multijuga Benth. seeds were found to contain protein (364 mg g−1 dwt), lipids (24 mg g−1 dwt), ash (35 mg g−1 dwt), and carbohydrates (577 mg g−1 dwt). Sucrose, raffinose, and stachyose concentrations were 8.3, 3.0, and 11.6 mg g−1 dwt, respectively. α-Galactosidase activity increased during seed germination and reached a maximum level at 108 h after seed imbibition. The α-galactosidase purified from germinating seeds had an Mr of 38,000 and maximal activity at pH 5.0–5.5 and 50 °C. The enzyme was stable at 35 °C and 40 °C, but lost 79% of its activity after 30 min at 50 °C. The activation energy (Ea) values for p-nitrophenyl-α-d-galactopyranoside (pNPGal) and raffinose were 13.86 and 4.75 kcal mol−1, respectively. The Km values for pNPGal, melibiose, raffinose, and stachyose were 0.45, 5.37, 39.62 and 48.80 mM, respectively. The enzyme was sensitive to inhibition by HgCl2, SDS, AgNO3, CuSO4, and melibiose. d-Galactose was a competitive inhibitor (Ki = 2.74 mM). In addition to its ability to hydrolyze raffinose and stachyose, the enzyme also hydrolyzed galactomannan.
Palavras-chave: Tachigali multijuga benth
Fabaceae
Seed germination
α-Galactosidase
Galactomannans
Raffinose family oligosaccharides
Editor: Phytochemistry
Tipo de Acesso: Elsevier Ltd.
URI: https://doi.org/10.1016/j.phytochem.2008.08.017
http://www.locus.ufv.br/handle/123456789/19072
Data do documento: 2-Out-2008
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