Use este identificador para citar ou linkar para este item:
https://locus.ufv.br//handle/123456789/19072
Tipo: | Artigo |
Título: | Characterization and biotechnological application of an acid α-galactosidase from Tachigali multijuga Benth. seeds |
Autor(es): | Fialho, Lílian da Silva Guimarães, Valéria Monteze Callegari, Carina Marin Reis, Angélica Pataro Barbosa, Daianny Silveira Borges, Eduardo Euclydes de Lima Moreira, Maurilio Alves Rezende, Sebastião Tavares de |
Abstract: | Tachigali multijuga Benth. seeds were found to contain protein (364 mg g−1 dwt), lipids (24 mg g−1 dwt), ash (35 mg g−1 dwt), and carbohydrates (577 mg g−1 dwt). Sucrose, raffinose, and stachyose concentrations were 8.3, 3.0, and 11.6 mg g−1 dwt, respectively. α-Galactosidase activity increased during seed germination and reached a maximum level at 108 h after seed imbibition. The α-galactosidase purified from germinating seeds had an Mr of 38,000 and maximal activity at pH 5.0–5.5 and 50 °C. The enzyme was stable at 35 °C and 40 °C, but lost 79% of its activity after 30 min at 50 °C. The activation energy (Ea) values for p-nitrophenyl-α-d-galactopyranoside (pNPGal) and raffinose were 13.86 and 4.75 kcal mol−1, respectively. The Km values for pNPGal, melibiose, raffinose, and stachyose were 0.45, 5.37, 39.62 and 48.80 mM, respectively. The enzyme was sensitive to inhibition by HgCl2, SDS, AgNO3, CuSO4, and melibiose. d-Galactose was a competitive inhibitor (Ki = 2.74 mM). In addition to its ability to hydrolyze raffinose and stachyose, the enzyme also hydrolyzed galactomannan. |
Palavras-chave: | Tachigali multijuga benth Fabaceae Seed germination α-Galactosidase Galactomannans Raffinose family oligosaccharides |
Editor: | Phytochemistry |
Tipo de Acesso: | Elsevier Ltd. |
URI: | https://doi.org/10.1016/j.phytochem.2008.08.017 http://www.locus.ufv.br/handle/123456789/19072 |
Data do documento: | 2-Out-2008 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo.pdf Until 2100-12-31 | Texto completo | 689,03 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.