Use este identificador para citar ou linkar para este item:
https://locus.ufv.br//handle/123456789/19657
Tipo: | Artigo |
Título: | Galleria mellonella is an effective model to study Actinobacillus pleuropneumoniae infection |
Autor(es): | Pereira, Monalessa Fábia Rossi, Ciro César Queiroz, Marisa Vieira de Martins, Gustavo Ferreira Isaac, Clement Bossé, Janine T. Li, Yanwen Wren, Brendan W. Terra, Vanessa Sofia Cuccui, Jon Langford, Paul R. Bazzolli, Denise Mara Soares |
Abstract: | Actinobacillus pleuropneumoniae is responsible for swine pleuropneumonia, a respiratory disease that causes significant global economic loss. Its virulence depends on many factors, such as capsular polysaccharides, RTX toxins and iron-acquisition systems. Analysis of virulence may require easy-to-use models that approximate mammalian infection and avoid ethical issues. Here, we investigate the potential use of the wax moth Galleria mellonella as an informative model for A. pleuropneumoniae infection. Genotypically distinct A. pleuropneumoniae clinical isolates were able to kill larvae at 37 6C but had different LD 50 values, ranging from 10 4 to 10 7 c.f.u. per larva. The most virulent isolate (1022) was able to persist and replicate within the insect, while the least virulent (780) was rapidly cleared. We observed a decrease in haemocyte concentration, aggregation and DNA damage post-infection with isolate 1022. Melanization points around bacterial cells were observed in the fat body and pericardial tissues of infected G. mellonella, indicating vigorous cell and humoral immune responses close to the larval dorsal vessel. As found in pigs, an A. pleuropneumoniae hfq mutant was significantly attenuated for infection in the G. mellonella model. Additionally, the model could be used to assess the effectiveness of several antimicrobial agents against A. pleuropneumoniae in vivo. G. mellonella is a suitable inexpensive alternative infection model that can be used to study the virulence of A. pleuropneumoniae, as well as assess the effectiveness of antimicrobial agents against this pathogen. |
Palavras-chave: | Galleria mellonella Actinobacillus pleuropneumoniae |
Editor: | Microbiology |
Tipo de Acesso: | Microbiology Society |
URI: | https://doi.org/10.1099/mic.0.083923-0 http://www.locus.ufv.br/handle/123456789/19657 |
Data do documento: | 14-Nov-2014 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo.pdf | Texto completo | 1,68 MB | Adobe PDF | Visualizar/Abrir |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.