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https://locus.ufv.br//handle/123456789/19690
Tipo: | Artigo |
Título: | Candidate gene expression and intramuscular fat content in pigs |
Autor(es): | Serão, N.V.L. Veroneze, R. Ribeiro, A.M.F. Verardo, L.L. Braccini Neto, J. Gasparino, E. Campos, C.F. Lopes, P.S. Guimarães, S.E.F. |
Abstract: | Seventy-two pigs of three genetic groups (Brazilian indigenous breed Piau, Commercial line and Crossbred) of both sexes were slaughtered at four live weights (30, 60, 90 and 120 kg). Intramuscular fat (IMF) content in Longissimus dorsi muscle of each animal was extracted and correlated with candidate gene mRNA expression (ATN1, EEF1A2, FABP3, LDLR, MGP, OBSCN, PDHB, TRDN and RYR1). Within slaughter weight of 120 kg, Piau and Crossbred pigs showed higher IMF content (p < 0.05) than commercial animals, with 2.48, 2.08 and 1.00% respectively. Barrows presented higher values of IMF (p < 0.05) than gilts (1.54 and 1.30% respectively). Gene expression of EEF1A2, FABP3, LDLR, OBSCN, PDHB, TRDN and RYR1 were correlated with IMF (p < 0.05) using the whole dataset. For Piau data only, expression of FABP3, LDLR, MGP, OBSCN, PDHB, TRDN and RYR1 showed correlation with IMF (p < 0.05). Genes that have important roles in lipid transportation inside the cell (FABP3) and tissues (LDLR) showed correlation with IMF of, respectively, 0.68 and 0.63 using the whole data set, and 0.90 and 0.91 using data from Piau animals. The highly positive correlation of the LDLR and FAPB3 expression with IMF content may confirm that these genes are important for fat deposition in the porcine L. dorsi muscle. |
Palavras-chave: | Piau pig breed Pig genetics Pig production Real time quantitative PCR |
Editor: | Journal of Animal Breeding and Genetics |
Tipo de Acesso: | Open Access |
URI: | https://doi.org/10.1111/j.1439-0388.2010.00887.x http://www.locus.ufv.br/handle/123456789/19690 |
Data do documento: | 17-Jun-2010 |
Aparece nas coleções: | Artigos |
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artigo.pdf Until 2100-12-31 | Texto completo | 172,8 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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