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https://locus.ufv.br//handle/123456789/19902
Tipo: | Artigo |
Título: | Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides |
Autor(es): | Rezende, Sebastião T. de Marques, Virgínia M. Trevizano, Larissa M. Passos, Flávia M. L. Oliveira, Maria G. A. Bemquerer, Marcelo P. Oliveira, Jamil S. Guimarães, Valéria M. Viana, Pollyanna A. |
Abstract: | Raffinose oligosaccharides (RO) are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. ROs are hydrolyzed by α-galactosidases that cleave α-1,6-linkages of α-galactoside residues. The objectives of this study were the purification and characterization of extracellular α-galactosidase from Debaryomyces hansenii UFV-1. The enzyme purified by gel filtration and anion exchange chromatographies presented an Mr value of 60 kDa and the N-terminal amino acid sequence YENGLNLVPQMGWN. The Km values for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.30, 2.01, 9.66, and 16 mM, respectively. The α-galactosidase presented absolute specificity for galactose in the α-position, hydrolyzing pNPGal, stachyose, raffinose, melibiose, and polymers. The enzyme was noncompetitively inhibited by galactose (Ki = 2.7 mM) and melibiose (Ki = 1.2 mM). Enzyme treatments of soy milk for 4 h at 60 °C reduced the amounts of stachyose and raffinose by 100%. |
Palavras-chave: | α-Galactosidase Debaryomyces hansenii UFV-1 Raffinose oligosaccharides Characterization Flatulence |
Editor: | Journal of Agricultural and Food Chemistry |
Tipo de Acesso: | American Chemical Society |
URI: | http://dx.doi.org/10.1021/jf0526442 http://www.locus.ufv.br/handle/123456789/19902 |
Data do documento: | 17-Fev-2006 |
Aparece nas coleções: | Artigos |
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artigo.pdf Until 2100-12-31 | texto completo | 182,4 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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