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https://locus.ufv.br//handle/123456789/22116
Tipo: | Artigo |
Título: | Virus-induced gene silencing of pea CHLI and CHLD affects tetrapyrrole biosynthesis, chloroplast development and the primary metabolic network |
Autor(es): | Araújo, Wagner Luiz Luo, Tao Luo, Sha Schlicke, Hagen Rothbart, Maxi Yu, Jing Fan, Tingting Fernie, Alisdair R. Grimm, Bernhard Luo, Meizhong |
Abstract: | The first committed and highly regulated step of chlorophyll biosynthesis is the insertion of Mg2+ into protoporphyrin IX, which is catalyzed by Mg chelatase that consists of CHLH, CHLD and CHLI subunits. In this study, CHLI and CHLD genes were suppressed by virus-induced gene silencing (VIGS-CHLI and VIGS-CHLD) in pea (Pisum sativum), respectively. VIGS-CHLI and VIGS-CHLD plants both showed yellow leaf phenotypes with the reduced Mg chelatase activity and the inactivated synthesis of 5-aminolevulinic acid. The lower chlorophyll accumulation correlated with undeveloped thylakoid membranes, altered chloroplast nucleoid structure, malformed antenna complexes and compromised photosynthesis capacity in the yellow leaf tissues of the VIGS-CHLI and VIGS-CHLD plants. Non-enzymatic antioxidant contents and the activities of antioxidant enzymes were altered in response to enhanced accumulation of reactive oxygen species (ROS) in the chlorophyll deficient leaves of VIGS-CHLI and VIGS-CHLD plants. Furthermore, the results of metabolite profiling indicate a tight correlation between primary metabolic pathways and Mg chelatase activity. We also found that CHLD induces a feedback-regulated change of the transcription of photosynthesis-associated nuclear genes. CHLD and CHLI silencing resulted in a rapid reduction of photosynthetic proteins. Taken together, Mg chelatase is not only a key regulator of tetrapyrrole biosynthesis but its activity also correlates with ROS homeostasis, primary interorganellar metabolism and retrograde signaling in plant cells. |
Palavras-chave: | Metabolite profiling Mg chelatase subunit CHLD Mg chelatase subunit CHLI Pisum sativum Tetrapyrrole biosynthesis Virus-induced gene silencing |
Editor: | Plant Physiology and Biochemistry |
Tipo de Acesso: | Elsevier B. V. |
URI: | https://doi.org/10.1016/j.plaphy.2013.01.006 http://www.locus.ufv.br/handle/123456789/22116 |
Data do documento: | Abr-2013 |
Aparece nas coleções: | Artigos |
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artigo.pdf | Texto completo | 1,29 MB | Adobe PDF | Visualizar/Abrir |
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