Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/1084
Tipo: Tese
Título: Cultivo in vitro de orquídeas do grupo Cattleya, em diferentes meios de cultura e irradiâncias
Título(s) alternativo(s): Orchids cultivation in vitro of the Cattleya group, in different means of culture and light irradiations
Autor(es): Ventura, Gizella Machado
Primeiro Orientador: Dias, José Maria Moreira
Primeiro coorientador: Novais, Roberto Ferreira de
Segundo coorientador: Cecon, Paulo Roberto
Primeiro avaliador: Venegas, Victor Hugo Alvarez
Segundo avaliador: Faria, Ricardo Tadeu de
Terceiro avaliador: Alexandre, Rodrigo Sobreira
Abstract: The propagation in vitro of orchids requires specific conditions to be cultivated. This work aimed to evaluate the effects of the methods of cultivation in the process of germination and growth of the species of the Cattleya group, and establish better levels of irradiation for the growth of vitroplants. We evaluated the effects of adding doses (0,0; 1,0; 2,0; 3,0 e 4,0 g L-1) of activated charcoal and of commercial brands in the means of culture GB5, MN, and Peter s. In the second step we evaluated four formulations in the means of culture (Peter s; MS; GB5; and B&G), with 0,0 and 2,0 g L-1 activated charcoal. Finally we studied the effects of the light irradiation (12; 25; 50; 100; and 150 µmol m-2 s-1). The seeds germination of the Laelia purpurata var. carnea, Cattleya amethystoglossa e Laelia anceps var. semia-alba occurred in less time (≈ 30 days) and in higher percentage level (80%) in means of culture without the addition of activated charcoal. In the same conditions, there were more green protocorms also, but the addition of activated charcoal increased the growth of the protocorms with roots. Simpler formulations, for example, the commercial fertilizer Peter s or B&G orchidée can substitute more complex means of culture as MS (1962) and GB5 (1968). Vitroplants of the Cattleya guttata Leopoldi x Lc. Guadalajara cultivated under light irradiation of the 65 µmol m-2 s-1 resulted in a better vegetative growth. In higher levels of irradiation, photo inhibitor effects were observed, but it was reversible in lower frequency, showing the plasticity of the hybrid studied.
The propagation in vitro of orchids requires specific conditions to be cultivated. This work aimed to evaluate the effects of the methods of cultivation in the process of germination and growth of the species of the Cattleya group, and establish better levels of irradiation for the growth of vitroplants. We evaluated the effects of adding doses (0,0; 1,0; 2,0; 3,0 e 4,0 g L-1) of activated charcoal and of commercial brands in the means of culture GB5, MN, and Peter s. In the second step we evaluated four formulations in the means of culture (Peter s; MS; GB5; and B&G), with 0,0 and 2,0 g L-1 activated charcoal. Finally we studied the effects of the light irradiation (12; 25; 50; 100; and 150 µmol m-2 s-1). The seeds germination of the Laelia purpurata var. carnea, Cattleya amethystoglossa e Laelia anceps var. semia-alba occurred in less time (≈ 30 days) and in higher percentage level (80%) in means of culture without the addition of activated charcoal. In the same conditions, there were more green protocorms also, but the addition of activated charcoal increased the growth of the protocorms with roots. Simpler formulations, for example, the commercial fertilizer Peter s or B&G orchidée can substitute more complex means of culture as MS (1962) and GB5 (1968). Vitroplants of the Cattleya guttata Leopoldi x Lc. Guadalajara cultivated under light irradiation of the 65 µmol m-2 s-1 resulted in a better vegetative growth. In higher levels of irradiation, photo inhibitor effects were observed, but it was reversible in lower frequency, showing the plasticity of the hybrid studied.
Palavras-chave: Cultura de tecidos
Germinação
Protocormos
Plant tissue culture
Germination
CNPq: CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
Idioma: por
País: BR
Editor: Universidade Federal de Viçosa
Sigla da Instituição: UFV
Departamento: Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de
Programa: Doutorado em Fitotecnia
Citação: VENTURA, Gizella Machado. Orchids cultivation in vitro of the Cattleya group, in different means of culture and light irradiations. 2007. 122 f. Tese (Doutorado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2007.
Tipo de Acesso: Acesso Aberto
URI: http://locus.ufv.br/handle/123456789/1084
Data do documento: 5-Jul-2007
Aparece nas coleções:Fitotecnia

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