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https://locus.ufv.br//handle/123456789/1084
Tipo: | Tese |
Título: | Cultivo in vitro de orquídeas do grupo Cattleya, em diferentes meios de cultura e irradiâncias |
Título(s) alternativo(s): | Orchids cultivation in vitro of the Cattleya group, in different means of culture and light irradiations |
Autor(es): | Ventura, Gizella Machado |
Primeiro Orientador: | Dias, José Maria Moreira |
Primeiro coorientador: | Novais, Roberto Ferreira de |
Segundo coorientador: | Cecon, Paulo Roberto |
Primeiro avaliador: | Venegas, Victor Hugo Alvarez |
Segundo avaliador: | Faria, Ricardo Tadeu de |
Terceiro avaliador: | Alexandre, Rodrigo Sobreira |
Abstract: | The propagation in vitro of orchids requires specific conditions to be cultivated. This work aimed to evaluate the effects of the methods of cultivation in the process of germination and growth of the species of the Cattleya group, and establish better levels of irradiation for the growth of vitroplants. We evaluated the effects of adding doses (0,0; 1,0; 2,0; 3,0 e 4,0 g L-1) of activated charcoal and of commercial brands in the means of culture GB5, MN, and Peter s. In the second step we evaluated four formulations in the means of culture (Peter s; MS; GB5; and B&G), with 0,0 and 2,0 g L-1 activated charcoal. Finally we studied the effects of the light irradiation (12; 25; 50; 100; and 150 µmol m-2 s-1). The seeds germination of the Laelia purpurata var. carnea, Cattleya amethystoglossa e Laelia anceps var. semia-alba occurred in less time (≈ 30 days) and in higher percentage level (80%) in means of culture without the addition of activated charcoal. In the same conditions, there were more green protocorms also, but the addition of activated charcoal increased the growth of the protocorms with roots. Simpler formulations, for example, the commercial fertilizer Peter s or B&G orchidée can substitute more complex means of culture as MS (1962) and GB5 (1968). Vitroplants of the Cattleya guttata Leopoldi x Lc. Guadalajara cultivated under light irradiation of the 65 µmol m-2 s-1 resulted in a better vegetative growth. In higher levels of irradiation, photo inhibitor effects were observed, but it was reversible in lower frequency, showing the plasticity of the hybrid studied. The propagation in vitro of orchids requires specific conditions to be cultivated. This work aimed to evaluate the effects of the methods of cultivation in the process of germination and growth of the species of the Cattleya group, and establish better levels of irradiation for the growth of vitroplants. We evaluated the effects of adding doses (0,0; 1,0; 2,0; 3,0 e 4,0 g L-1) of activated charcoal and of commercial brands in the means of culture GB5, MN, and Peter s. In the second step we evaluated four formulations in the means of culture (Peter s; MS; GB5; and B&G), with 0,0 and 2,0 g L-1 activated charcoal. Finally we studied the effects of the light irradiation (12; 25; 50; 100; and 150 µmol m-2 s-1). The seeds germination of the Laelia purpurata var. carnea, Cattleya amethystoglossa e Laelia anceps var. semia-alba occurred in less time (≈ 30 days) and in higher percentage level (80%) in means of culture without the addition of activated charcoal. In the same conditions, there were more green protocorms also, but the addition of activated charcoal increased the growth of the protocorms with roots. Simpler formulations, for example, the commercial fertilizer Peter s or B&G orchidée can substitute more complex means of culture as MS (1962) and GB5 (1968). Vitroplants of the Cattleya guttata Leopoldi x Lc. Guadalajara cultivated under light irradiation of the 65 µmol m-2 s-1 resulted in a better vegetative growth. In higher levels of irradiation, photo inhibitor effects were observed, but it was reversible in lower frequency, showing the plasticity of the hybrid studied. |
Palavras-chave: | Cultura de tecidos Germinação Protocormos Plant tissue culture Germination |
CNPq: | CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA |
Idioma: | por |
País: | BR |
Editor: | Universidade Federal de Viçosa |
Sigla da Instituição: | UFV |
Departamento: | Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de |
Citação: | VENTURA, Gizella Machado. Orchids cultivation in vitro of the Cattleya group, in different means of culture and light irradiations. 2007. 122 f. Tese (Doutorado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2007. |
Tipo de Acesso: | Acesso Aberto |
URI: | http://locus.ufv.br/handle/123456789/1084 |
Data do documento: | 5-Jul-2007 |
Aparece nas coleções: | Fitotecnia |
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