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Campo DC | Valor | Idioma |
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dc.contributor.author | Almeida, Maíra N.de | |
dc.contributor.author | Falkoski, Daniel L. | |
dc.contributor.author | Guimarães, Valéria M. | |
dc.contributor.author | Ramos, Humberto Josué de O. | |
dc.contributor.author | Visser, Evan M. | |
dc.contributor.author | Maitan-Alfenas, Gabriela P. | |
dc.contributor.author | Rezende, Sebastião T. de | |
dc.date.accessioned | 2018-03-08T14:53:40Z | |
dc.date.available | 2018-03-08T14:53:40Z | |
dc.date.issued | 2013-06-08 | |
dc.identifier.issn | 09608524 | |
dc.identifier.uri | https://doi.org/10.1016/j.biortech.2013.06.021 | |
dc.identifier.uri | http://www.locus.ufv.br/handle/123456789/18128 | |
dc.description.abstract | A novel multienzyme complex, E1 C , and a free endoglucanase, E2 (GH5), from Fusarium verticillioides were purified. The E1 C contained two endoglucanases (GH6 and GH10), one cellobiohydrolase (GH7) and one xylanase (GH10). Maximum activity was observed at 80 °C for both enzymes and they were thermostable at 50 and 60 °C. The activation energies for E1 C and E2 were 21.3 and 27.5 kJ/mol, respectively. The K M for E1 C was 10.25 g/L while for E2 was 6.58 g/L. Both E1 C and E2 were activated by Mn 2+ and CoCl 2 while they were inhibited by SDS, CuSO 4 , FeCl 3 , AgNO 4 , ZnSO 4 and HgCl 2 . E1 C and E2 presented endo-b-1,3–1,4-glucanase activity. E1 C presented crescent activity towards cellopentaose, cellotetraose and cellotriose. E2 hydrolyzed the substrates cellopentaose, cellotetraose and cellotriose with the same efficiency. E1 C showed a higher stability and a better hydrolysis performance than E2, suggesting advantages resulting from the physical interaction between proteins. | en |
dc.format | pt-BR | |
dc.language.iso | eng | pt-BR |
dc.publisher | Bioresource Technology | pt-BR |
dc.relation.ispartofseries | v. 143, p. 413-422, September 2013 | pt-BR |
dc.rights | Published by Elsevier Ltd. | pt-BR |
dc.subject | Multienzyme complex | pt-BR |
dc.subject | Endoglucanase | pt-BR |
dc.subject | Cellobiohydrolase | pt-BR |
dc.subject | Xylanase | pt-BR |
dc.subject | Fusarium verticillioides | pt-BR |
dc.title | Characteristics of free endoglucanase and glycosidases multienzyme complex from Fusarium verticillioides | en |
dc.type | Artigo | pt-BR |
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artigo.pdf Until 2100-12-31 | Texto completo | 1,05 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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