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https://locus.ufv.br//handle/123456789/18293
Tipo: | Artigo |
Título: | Influence of the preservation period in silica-gel on the predatory activity of the isolates of Duddingtonia flagrans on infective larvae of cyathostomins (Nematoda: Cyathostominae) |
Autor(es): | Braga, Fabio Ribeiro Araújo, Jackson Victor Araujo, Juliana Milani Tavela, Alexandre de Oliveira Ferreira, Sebastião Rodrigo Soares, Filippe E. Freitas Benjamin, Laércio dos Anjos Frassy, Luiza Neme |
Abstract: | The continued maintenance of nematophagous fungi predatory activity under laboratory conditions is one of the basic requirements for a successful biological control. The purpose of this study was to evaluate the influence of time on the preservation of the fungus Duddingtonia flagrans (AC001 and CG722) stored in silica-gel for 7 years and their subsequent predatory activity on cyathostomin L3 larvae in 2% water-agar medium (2% WA). Samples of the isolates AC001 and CG722, originating from vials containing grains of silica-gel sterilized and stored for 7 years, were used. After obtaining fungal conidia, the predation test was conducted over 7 days on the surface of 9.0 cm Petri dishes filled with 2% WA. In the treated groups each Petri dish contained 500 cyathostomin L3 and conidia of fungal isolates in 2% WA. In the control group (without fungi) the plates contained 500 L3 in 2% WA. The experimental results showed that isolated AC001 and CG722 were efficient in preying on cyathostomin L3 (p < 0.01) compared to control (without fungus). However, no difference was observed (p > 0.01) in the predatory activity of the fungal isolates tested. Comparing the groups, there was a significant reductions of cyathostomin L3 (p < 0.01) of 88.6% and 78.4% on average recovered from the groups treated with the isolates AC001 and CG722, respectively, after 7 days. The results of this test showed that the fungus D. flagrans (AC001 and CG722) stored in silica-gel for at least 7 years maintained its predatory activity on cyathostomin L3. |
Palavras-chave: | Nematophagous fungi Duddingtonia flagrans Silica-gel Cyathostomins |
Editor: | Experimental Parasitology |
Tipo de Acesso: | Elsevier Inc. |
URI: | https://doi.org/10.1016/j.exppara.2011.05.013 http://www.locus.ufv.br/handle/123456789/18293 |
Data do documento: | 24-Mai-2011 |
Aparece nas coleções: | Artigos |
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Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 662,95 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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