Use este identificador para citar ou linkar para este item:
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorBlanco, Luis Andrés Arteaga
dc.contributor.authorCrispim, Josicelli Souza
dc.contributor.authorFernandes, Kenner Morais
dc.contributor.authorOliveira, Leandro Licursi de
dc.contributor.authorPereira, Monalessa Fábia
dc.contributor.authorBazzolli, Denise Mara Soares
dc.contributor.authorMartins, Gustavo Ferreira
dc.description.abstractIn the present work, we have investigate the cellular immune response of Galleria mellonella larvae against three strains of the gram-negative bacterium Actinobacillus pleuropneumoniae: low-virulence (780), high-virulence (1022) and the serotype 8 reference strain (R8). Prohemocytes, plasmatocytes, granulocytes, oenocytoids and spherulocytes were distinguished according to their size and morphology, their molecular markers and dye-staining properties and their role in the immune response. Total hemocyte count, differential hemocyte count, lysosome activity, autophagic response, cell viability and caspase-3 activation were determined in circulating hemocytes of naive and infected larvae. The presence of the autophagosome protein LC3 A/B within the circulating hemocytes of G. mellonella was dependent on and related to the infecting A. pleuropneumoniae strain and duration of infection. Hemocytes treated with the high-virulence strain expressed higher levels of LC3 A/B, whereas treatment with the low-virulence strain induced lower expression levels of this protein in the cells. Moreover, our results showed that apoptosis in circulating hemocytes of G. mellonella larvae after exposure to virulent bacterial strains occurred simultaneously with excessive cell death response induced by stress and subsequent caspase-3 activation.en
dc.publisherCell and Tissue Researchpt-BR
dc.relation.ispartofseriesv. 370, Issue 1, p. 153–168, October 2017pt-BR
dc.rightsSpringer Berlin Heidelbergpt-BR
dc.subjectImmune responsept-BR
dc.subjectActinobacillus pleuropneumoniaept-BR
dc.subjectGalleria mellonellapt-BR
dc.titleDifferential cellular immune response of Galleria mellonella to Actinobacillus pleuropneumoniaeen
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
  Until 2100-12-31
Texto completo7,79 MBAdobe PDFVisualizar/Abrir ACESSO RESTRITO

Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.