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https://locus.ufv.br//handle/123456789/19662
Tipo: | Artigo |
Título: | Development of new molecular markers for the Colletotrichum genus using RetroCl1 sequences |
Autor(es): | Santos, Leandro Vieira dos Queiroz, Marisa Vieira de Santana, Mateus Ferreira Soares, Marcos Antonio Barros, Everaldo Goncalves de Araujo, Elza Fernandes de Langin, Thierry |
Abstract: | A nonautonomous element of 624 bp, called RetroCl1 (Retroelement Colletotrichum lindemuthianum 1), was identified in the plant pathogenic fungus Colletotrichum lindemuthianum. RetroCl1 contains terminal direct repeats (223 bp) that are surrounded by CTAGT sequences. It has a short internal domain of 178 bp and shows characteristics of terminal-repeat retrotransposon in miniature (TRIM) family. We used RetroCl1 sequence to develop molecular markers for the Colletotrichum genus. IRAP (Inter-Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism) markers were used to analyze the genetic diversity of C. lindemuthianum. Fifty-four isolates belonging to different races were used. A total of 45 loci were amplified. The Nei index showed significant differences among the populations divided according to race, indicating that they are structured according to pathotype. No clear correlation between IRAP and REMAP markers with pathogenic characterization was found. C. lindemuthianum has high genetic diversity, and the analysis of molecular variance showed that 51% of variability is found among the populations of different races. The markers were also tested in different Colletotrichum species. In every case, multiple bands were amplified, indicating that these markers can be successfully used in different species belonging to the Colletotrichum genus. |
Palavras-chave: | TRIM Retrotransposon IRAP REMAP Anthracnose |
Editor: | World Journal of Microbiology and Biotechnology |
Tipo de Acesso: | Springer Science+Business Media B.V. |
URI: | http://dx.doi.org/10.1007/s11274-011-0909-x http://www.locus.ufv.br/handle/123456789/19662 |
Data do documento: | 1-Out-2011 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | texto completo | 406,2 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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