Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19923
Tipo: Artigo
Título: Combinatorial regulation modules on GmSBP2 promoter: a distal cis-regulatory domain confines the SBP2 promoter activity to the vascular tissue in vegetative organs
Autor(es): Waclawovsky, Alessandro J.
Freitas, Rejane L.
Rocha, Carolina S.
Contim, Luis Antônio S.
Fontes, Elizabeth P.B.
Abstract: The Glycine max sucrose binding protein (GmSBP2) promoter directs phloem-specific expression of reporter genes in transgenic tobacco. Here, we identified cis-regulatory domains (CRD) that contribute with positive and negative regulation for the tissue-specific pattern of the GmSPB2 promoter. Negative regulatory elements in the distal CRD-A (−2000 to −700) sequences suppressed expression from the GmSBP2 promoter in tissues other than seed tissues and vascular tissues of vegetative organs. Deletion of this region relieved repression resulting in a constitutive promoter highly active in all tissues analyzed. Further deletions from the strong constitutive −700GmSBP2 promoter delimited several intercalating enhancer-like and repressing domains that function in a context-dependent manner. Histochemical examination revealed that the CRD-C (−445 to −367) harbors both negative and positive elements. This region abolished promoter expression in roots and in all tissues of stems except for the inner phloem. In contrast, it restores root meristem expression when fused to the −132pSBP2-GUS construct, which contains root meristem expression-repressing determinants mapped to the 44-bp CRD-G (−136 to −92). Thus, the GmSBP2 promoter is functionally organized into a proximal region with the combinatorial modular configuration of plant promoters and a distal domain, which restricts gene expression to the vascular tissues in vegetative organs.
Palavras-chave: Sucrose binding protein
Phloem-specific expression
GmSBP
Soybean
Tissue-specificity, promoter activity
Editor: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
Tipo de Acesso: Elsevier B.V.
URI: https://doi.org/10.1016/j.bbaexp.2006.02.002
http://www.locus.ufv.br/handle/123456789/19923
Data do documento: 10-Mar-2006
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
artigo.pdf
  Until 2100-12-31
texto completo725,56 kBAdobe PDFVisualizar/Abrir    Solictar uma cópia


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.