Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/22774
Tipo: Artigo
Título: Utilization of phage display to identify antigenic regions in the PCV2 capsid protein for the evaluation of serological responses in mice and pigs
Autor(es): Santos, Marcus Rebouças
Assao, Viviane Sisdelli
Salgado, Rafael Locatelli
Fietto, Juliana Lopes Rangel
Bressan, Gustavo Costa
Almeida, Márcia Rogéria de
Goulart, Luíz Ricardo
Silva-Júnior, Abelardo
Santos, Fabiana de Almeida Araújo
Carneiro, Ana Paula
Lobato, Zelia Inês Portela
Veira, Carlos Ueira
Abstract: Porcine circovirus 2 (PCV2) is associated with a series of swine diseases. There is a great interest in improving our understanding of the immunology of PCV2, especially the properties of the viral capsid protein Cap-PCV2 and how they relate to the immunogenicity of the virus and the subsequent development of vaccines. Phage display screening has been widely used to study binding affinities for target proteins. The aim of this study was to use phage display screening to identify antigenic peptides in the PCV2 capsid protein. After the selection of peptides, five of them presented similarity to sequences found in cap-PCV2, and four peptides were synthesized and used for immunization in mice: 51–CTFGYTIKRTVT-62 (PS14), 127-CDNFVTKATALTY-138 (PS34), 164-CKPVLDSTIDY-173 (PC12), and 79-CFLPPGGGSNT-88 (PF1). Inoculation with the PC12 peptide led to the highest production of antibodies. Furthermore, we used the PC12 peptide as an antigen to examine the humoral response of swine serum by ELISA. The sensitivity and specificity of this assay was 88.9% and 92.85%, respectively. Altogether, characterization of immunogenic epitopes in the capsid protein of PCV2 may contribute to the improvement of vaccines and diagnostics.
Palavras-chave: Identify antigenic
PCV2 capsid protein
Evaluation of serological
Mice and pigs
Editor: Archives of Virology
Tipo de Acesso: Springer-Verlag GmbH Austria, part of Springer Nature 2018
URI: https://doi.org/10.1007/s00705-018-3816-8
http://www.locus.ufv.br/handle/123456789/22774
Data do documento: Jul-2018
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